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标题: 求助CELL最新文章Control of the embryonic stem cell state [打印本页]

作者: clairezy1983 时间: 2011-4-8 15:32 标题: 求助CELL最新文章Control of the embryonic stem cell state

本帖最后由 clairezy1983 于 2011-4-8 15:57 编辑 ! M6 @; n& x, W# v

Cell. 2011 Mar 18;144(6):940-54.
Control of the embryonic stem cell state.7 ]* ]. \9 B2 E' r- J
Young RA.& e4 ^6 Q* s- @7 P$ }- J
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA./ Z7 x: w7 W" [( z5 {
Abstract
Embryonic stem cells and induced pluripotent stem cells hold great promise for regenerative medicine. These cells can be propagated in culture in an undifferentiated state but can be induced to differentiate into specialized cell types. Moreover, these cells provide a powerful model system for studies of cellular identity and early mammalian development. Recent studies have provided insights into the transcriptional control of embryonic stem cell state, including the regulatory circuitry underlying pluripotency. These studies have, as a consequence, uncovered fundamental mechanisms that control mammalian gene expression, connect gene expression to chromosome structure, and contribute to human disease.6 L8 R# K7 U3 S
Copyright © 2011 Elsevier Inc. All rights reserved.) W8 R& G! e {
PMID: 21414485 [PubMed - in process]- T: @" \8 z- a+ ~

作者: clairezy1983 时间: 2011-4-8 15:34

顶起来！我们的图书馆啊，伤心，最新的文献都下不到，复印要4毛一张，小女子囊中羞涩啊，求助各位大虾了

作者: clairezy1983 时间: 2011-4-8 15:50

再求助一篇啊* M' T6 r- b6 q4 M; n9 K

J Cell Biochem. 2011 Mar 17. doi: 10.1002/jcb.23106. [Epub ahead of print]- n4 P2 {9 R% e
MicroRNA expression during osteogenic differentiation of human multipotent mesenchymal stromal cells from bone marrow.+ F3 A$ c3 u( k% u% k3 @9 d
Gao J, Yang T, Han J, Yan K, Qiu X, Zhou Y, Fan Q, Ma B.
Department of Orthopedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi, 710038, P. R. China.' p" F: a& M& w( N u
Abstract- m. O1 I( L0 q" P8 T/ R
MicroRNAs comprise a group of non-coding small RNAs (17-25 nt) involved in post-transcriptional regulation that have been identified in various plants and animals. Studies have demonstrated that miRNAs are associated with stem cell self-renewal and differentiation and play a key role in controlling stem cell activities. However, the identification of specific miRNAs and their regulatory roles in the differentiation of multipotent mesenchymal stromal cells (MSCs) have so far been poorly defined. We isolated and cultured human MSCs and osteo-differentiated MSCs from four individual donors. miRNA expression in MSCs and osteo-differentiated MSCs was investigated using miRNA microarrays. miRNAs that were commonly expressed in all three MSC preparations and miRNAs that were differentially expressed between MSCs and osteo-differentiated MSCs were identified. Four underexpressed (hsa-miR-31, hsa-miR-106a, hsa-miR-148a, and hsa-miR-424) and three novel overexpressed miRNAs (hsa-miR-30c, hsa-miR-15b and hsa-miR-130b) in osteo-differentiated MSCs were selected and their expression were verified in samples from the fourth individual donors. The putative targets of the miRNAs were predicted using bioinformatic analysis. The four miRNAs that were underexpressed in osteo-differentiated MSCs were predicted to target RUNX2, CBFB and BMPs, which are involved in bone formation; while putative targets for miRNAs overexpressed in osteo-differentiated MSCs were MSC maker(e.g. CD44, ITGB1, and FLT1), stemness-maintaining factor(e.g. FGF2 and CXCL12 ), and genes related to cell differentiation(e.g. BMPER, CAMTA1 and GDF6). Finally, hsa-miR-31 was selected for target verification and function analysis. The results of this study provide an experimental basis for further research on miRNA functions during osteogenic differentiation of human MSCs. © 2011 Wiley-Liss, Inc.
Copyright © 2011 Wiley-Liss, Inc.
PMID: 21416501 [PubMed - as supplied by publisher]2 w7 |1 V7 x5 z1 C" r$ C, A0 b
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作者: shaaou 时间: 2011-4-8 23:33

版主，大家都实在太热情了，有木有？！！！# e7 ?# m# P+ c* B
我想帮人都找不到机会，有木有？！！！) T; N4 d: t* m- D/ z
注册都好几年了还是新手上路，菜鸟真的伤不起啊。。。。

作者: shaaou 时间: 2011-4-8 23:34 标题: RE: 求助CELL最新文章Control of the embryonic stem cell state。

继续咆哮中。。。

作者: clairezy1983 时间: 2011-4-9 13:22

万分感激啊，真是好人多多啊！我喜欢干细胞之家，以后就在这里扎根啦！

作者: clairezy1983 时间: 2011-4-9 13:23

回复 shaaou 的帖子
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谢谢战友shaaou,谢谢谢谢啦

作者: puppypuppy 时间: 2011-12-16 22:13

哇晒，真好哦，我需要这个文献哦。

作者: cbcbcs 时间: 2011-12-27 19:08

没有包包。。。。。。

作者: webber43 时间: 2011-12-27 19:15

回复 clairezy1983 的帖子

开始看标题还突然兴奋了一下，原来是3月份的文章，不新了啊

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