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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑
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附加上目录，以供需要的人参考。
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+ P% T7 H- W [, S! hContents! o# [( `3 Z" t& t
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Preface ........................................................................................................v
! ]# r* j4 q3 ?Contributors ............................................................................................ xiii
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/ p3 b4 P8 y4 q0 IPART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES6 r" k1 p/ a6 G4 Z3 T) V
1 Transduction Technologies6 I0 n, L0 x' z" t* K- m$ s% s% i
David A. Fluri and Martin Fussenegger ........................................... 3' g9 _8 Z* f4 \
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,
9 K/ k6 H- j# ^8 C, |. l3 k- |and Otto-Wilhelm Merten ........................................................ 237 V% X' Z" u, Q# A
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
6 s* V; U* z7 K) ZAlexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93% o1 y& z g' c+ @, s h) @
4 DNA Fingerprinting and Characterization of Animal Cell Lines
+ D2 g; ~/ W& K, `: D- EGlyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123# B C$ s5 x; }2 }% p. n1 {2 B3 a* a
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PART II. BASIC CULTIVATION TECHNIQUES# L/ x2 e _( E7 K6 J4 h3 s* C
5 Microcarrier Cell Culture Technology c: T# l2 _3 d# a- z
Gerald Blüml ............................................................................... 149
) e$ `; r" ?) B5 ]( m! L9 S6 Cell Encapsulation
9 C1 d# P2 e+ g) q8 h3 RHolger Hübner ............................................................................ 179+ J' J r3 ~7 K3 \% d' f& Z; H
7 Tools for High-Throughput Medium and Process Optimization
8 c8 B O- A7 C; Z6 fMartin Jordan and Nigel Jenkins ................................................. 193- G9 a. X- ^5 c6 P' i9 |
) s, k8 _9 {) q' V) DPART III. CELL CHARACTERIZATION AND ANALYSIS% A- J5 g: _" x0 n( j7 c' a
8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205# ~0 N' M- u' Y# |; F
9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry4 L9 f0 V! U! k
Silvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 2232 ~. A$ r) N3 Y* [$ j
10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
$ h2 M# b) m4 H) O; C- B( o9 E/ ZAndré A. Neves and Kevin M. Brindle ........................................ 239
& |/ N$ s2 M% w' h11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
! F4 e3 o8 c9 [' Q& @" p( ^Heino Büntemeyer....................................................................... 253( O- r# X. g4 F5 ~
12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation
6 n; u" w+ q0 p2 J* i9 ~5 f3 @' ODerek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269" y! b3 [# v2 M$ d
13 Measurement of Apoptosis in Cell Culture8 [# I, G x* \) D7 h# W
Adiba Ishaque and Mohamed Al-Rubeai ..................................... 285! y) {7 O# s7 D9 y0 E8 B& {
14 Metabolic Flux Estimation in Mammalian Cell Cultures
, d, a2 g7 d" bChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 301, \8 T, i9 ^- w4 k
PART IV. SPECIAL CULTIVATION TECHNIQUES
/ ~6 g0 j2 X& J9 v+ r1 {6 m- S! p15 Disposable Bioreactors for Inoculum Production and Protein Expression
$ n9 e$ a9 m$ k) S9 `Regine Eibl and Dieter Eibl .......................................................... 321
W" b8 I8 v, C, T! v, H. H16 Hollow Fiber Cell Culture) o, Q6 z) P5 Y3 @! f! N7 {3 O/ T' p
John M. Davis .............................................................................. 337/ U% J* s; d% S5 l, A( }/ c( E- X! z
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors! X9 u8 u( u& X9 W
Ralf Pörtner and Oscar B. J. Platas Barradas .............................. 353
4 n* s% ?, F) U$ P3 x18 Configuration of Bioreactors0 a" x7 C; _# |2 B4 o- L t( ]
Dirk E. Martens and Evert Jan van den End ................................ 3717 p$ | b$ D$ n2 @8 }
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PART V. DOWNSTREAM TECHNIQUES5 N3 Z/ |" y" j7 f
19 Membrane Filtration in Animal Cell Culture* Q* o1 `6 L; A" @( y
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397& F1 }" {, W' C9 ~7 o* z
20 Chromatographic Techniques in the Downstream Processing0 m$ }; g, F$ h* x6 y# d
of (Recombinant) Proteins2 M* c2 K- T7 b2 a- z( K) l; U0 _, h
Ruth Freitag ................................................................................. 4216 J+ h2 k2 \& w0 k! d0 x0 F
PART VI. SPECIAL APPLICATIONS
0 _4 A- l5 l5 b; N }21 Vaccine Production: State of The Art and Future Needs
7 w: g ]+ z2 a- U3 _2 Jin Upstream Processing
: g$ ^ [: @) `6 JYvonne Genzel and Udo Reichl .................................................. 4570 V7 k3 }. K: _
22 Retrovirus Production and Characterization7 Q- o/ q- y. R6 q4 _! Q
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues,2 r+ f0 b$ Z$ b) R% v. @& v4 U7 @
and Paula Alves ....................................................................... 475
- R8 z) r1 ^# J' u0 I* j23 Insect Cell Cultivation and Generation of Recombinant8 E* J, p+ l& A- U
Baculovirus Particles for Recombinant Protein Production
" O6 R" G; h1 s' y$ ?1 fSabine Geisse ............................................................................... 4892 \* E3 p# h/ {/ i8 u
Index ...................................................................................................... 5099 R2 }+ F* y0 g: |) Q' b- F
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发表于 2011-3-31 12:51
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