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本帖最后由 细胞海洋 于 2011-6-11 14:46 编辑
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9 O( C8 u" @ w! U: L" d# `PCR Cloning Protocols
" T/ Y, G, \* M% s( i- gSecond Edition
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' e8 a: [ O( C) m. ]) oContents
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Preface .............................................................................................................v3 ^8 X9 ?0 m% p; j5 w, }
Contributors .....................................................................................................xi
- p1 A2 C f1 Q1 S7 V& xPART I. PERFORMING AND OPTIMIZING PCR
' E5 _- ?' s+ A" ?8 s1 Polymerase Chain Reaction: Basic Principles and Routine Practice
' D0 M9 t) b. D: m9 e' ELori A. Kolmodin and David E. Birch .................................................. 3
' @6 ~3 X4 g/ s9 ?6 W! L- a' E2 Computer Programs for PCR Primer Design and Analysis6 ~; v; b5 r" m) R5 \* F6 f
Bing-Yuan Chen, Harry W. Janes, and Steve Chen ........................ 19
# c9 Q9 I3 K7 H+ O/ V7 T6 F3 Single-Step PCR Optimization
; v2 Z4 F% k/ OUsing Touchdown and Stepdown PCR Programming
2 h+ f$ I; G0 ~4 O4 CKenneth H. Roux .................................................................................. 31
' e+ J2 m- P: G: ]' [4 XL PCR Amplification of Long Targets from Genomic DNA$ r* {0 g0 B( F1 A
Lori A. Kolmodin .................................................................................. 37* r A9 y3 K- A' p% `
5 Coupled One-Step Reverse Transcription and Polymerase Chain5 }6 a% _, X! b, O
Reaction Procedure for Cloning Large cDNA Fragments
0 B* Y% G( N4 C; U' Q) N& i/ ~5 e- a* uJyrki T. Aatsinki ................................................................................... 53
& J, ~, u7 i+ K5 Y l0 e6 Long Distance Reverse-Transcription PCR- ~8 p# A: D" b) o# I! I
Volker Thiel, Jens Herold, and Stuart G. Siddell ............................. 59
: j/ @1 [1 s: E# ?+ ^% V7 Increasing PCR Sensitivity for Amplification
7 o; } Z( ^. E& efrom Paraffin-Embedded Tissues, v- z4 p& {" K: p# _! j
Abebe Akalu and Juergen K. V. Reichardt ....................................... 67
6 T: P2 z l" m' F& A8 d @8 GC-Rich Template Amplification by Inverse PCR:9 y1 R J& C8 Q' A! z9 r B
DNA Polymerase and Solvent Effects" [& I% [ ~8 j' O6 y& m
Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez,; j1 t2 h+ ^1 ?* d& P& ?" {0 v
and Jean Dusart............................................................................... 75
, q0 M. X' L, K; H/ H9 PCR Procedure for the Isolation of Trinucleotide Repeats6 }# n5 L, L- i# G3 Z
Teruaki Tozaki ...................................................................................... 814 t! Y1 s: B/ L
10 Methylation-Specific PCR4 H: E& ?) \' a: ^
Haruhiko Ohashi .................................................................................. 91
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11 Direct Cloning of Full-Length Cell Differentially Expressed Genes
" U" l N+ W! G6 [! Sby Multiple Rounds of Subtractive Hybridization0 M" l; d0 N* p" N; O7 d* W; j, B
Based on Long-Distance PCR and Magnetic Beads
4 Z' S7 t+ Q3 y3 n! H% M9 j8 x' p- BXin Huang, Zhenglong Yuan, and Xuetao Cao ................................ 99
8 v. p8 i- x+ k' m8 s1 Q: DPART II. CLONING PCR PRODUCTS* h5 k& J T+ ]+ i% Q0 ]
12 Cloning PCR Products: An Overview
) J5 K C9 s% d( K9 LBaotai Guo and Yuping Bi ................................................................ 111
$ {, e: F# B3 {. p8 d13 Using T4 DNA Polymerase to Generate Clonable PCR Products
6 k2 u# Z2 I; x7 PKai Wang ............................................................................................. 121
, ]- m" b, @8 U5 B' h14 Enzyme-Free Cloning of PCR Products
) X4 D( H% T ~2 a0 `and Fusion Protein Expression: ~0 u) L. ~+ g: \( Q" V/ N& c
Brett A. Neilan and Daniel Tillett ..................................................... 125
, ^& p( }6 l! Q0 B0 r' Y3 b' V" {6 t15 Directional Restriction Site-Free Insertion of PCR Products
8 U! C: a8 H6 Uinto Vectors. ?) `5 G- x: B' p h* D3 N; f
Guo Jun Chen .................................................................................... 133
' r0 [! H3 B/ Q16 Autosticky PCR:" I, W. Z, j- X1 F7 x- E
Directional Cloning of PCR Products with Preformed 5' Overhangs; z6 G/ _. ~0 S4 T; c. q, w$ m
József Gál and Miklós Kálmán......................................................... 141: a( O8 v$ r6 {% l9 o5 ^
17 A Rapid and Simple Procedure for Direct Cloning
! J: h) j) [- R1 R3 ^of PCR Products into Baculoviruses
" Y# B' c: c3 ATamara S. Gritsun, Michael V. Mikhailov,1 N8 T; J4 z2 C! ^2 r3 n
and Ernest A. Gould ...................................................................... 153
0 V7 i! Y0 p1 L# z" B+ W" Q, ~PART III. MUTAGENESIS AND RECOMBINATION
3 Q' V, p. h7 t' z3 A9 g18 PCR Approaches to DNA Mutagenesis and Recombination:
* b5 _6 t1 B" X8 xAn Overview4 ^4 h1 {+ l& n
Binzhang Shen ................................................................................... 167
6 Q9 |6 G/ B0 [( v0 w* y* M19 In-Frame Cloning of Synthetic Genes Using PCR Inserts
5 N4 z# v2 i1 i+ C, U- O& O! i9 i! EJames C. Pierce ................................................................................. 175
! f4 _' D' u3 ^20 Megaprimer PCR
- ^3 {: Y4 |* G1 Z/ d5 ?Sailen Barik ........................................................................................ 189
! z5 z+ u- G' f) V# k6 E6 \3 { }6 [21 PCR-Mediated Recombination:
% W4 N# D9 R0 t3 g; xA General Method Applied to Construct Chimeric Infectious
. a* j7 J7 @( d' L' U/ K, T, w$ g7 iMolecular Clones3 d2 h# x- u% v, I; c
Guowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran,* ?3 Q! S0 n1 c* _* ~
and Harold Burger ......................................................................... 197
& e3 n! x. N, V4 f. f) l+ B7 R22 PCR Method for Generating Multiple Mutations at Adjacent Sites
( {/ S! U6 [5 X3 R! PJiri Adamec ......................................................................................... 207
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5 y8 ?- [! z, A- B1 y! r7 v4 D1 F6 }23 A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing$ h# D/ u' S. ~+ n9 ?% f' ]
Repeat Expansions into CAG-Repeat Containing Genes/ M: s/ R l. ?( @* B
Franco Laccone ................................................................................. 217) p/ j0 m+ m3 |2 Q5 O
24 PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
$ m# X# {1 l) h9 _, w( BDario E. Lehoux and Roger C. Levesque ....................................... 225
% L! @5 G. n" M" f! W7 e* V! X0 d25 Staggered Extension Process (StEP) In Vitro Recombination2 c' R5 c& U L& [3 }* @. m# U
Anna Marie Aguinaldo and Frances Arnold ................................... 235
7 H8 s5 {# M3 o/ _4 ^4 y26 Random Mutagenesis by Whole-Plasmid PCR Amplification
" r; F" t8 X7 E! O$ ^3 I: ^! xDonghak Kim and F. Peter Guengerich .......................................... 241
! L/ I) ^+ Q r1 fPART IV. CLONING UNKNOWN NEIGHBORING DNA1 e1 ?) w. W+ D
27 PCR-Based Strategies to Clone Unknown DNA Regions3 W0 q$ b' W% W% k0 M0 S4 H% ~
from Known Foreign Integrants: An Overview5 ~8 V/ ^% {, }
Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo ................ 249
9 g0 O; j( Q, x+ e. U3 H# b2 `28 Long Distance Vectorette PCR (LDV PCR)1 c$ F ]$ C( j
James A. L. Fenton, Guy Pratt, and Gareth J. Morgan ................. 275
6 R! M* ~. Y, e5 [' p29 Nonspecific, Nested Suppression PCR Method$ @5 m6 c8 B$ b6 B
for Isolation of Unknown Flanking DNA (“Cold-Start Method”)
, o- `2 ^/ ^; E* `: R6 w% hMichael Lardelli .................................................................................. 285
8 L" ~" C* w# a1 X30 Inverse PCR: cDNA Cloning
8 j8 a7 z, I# T3 g( R- ~Sheng-He Huang ................................................................................ 293
0 G8 r2 V) W2 o8 y31 Inverse PCR: Genomic DNA Cloning
" ]# x9 D# W N) D6 D- L0 E7 g: e( AAmbrose Y. Jong, Anna T’ang, De-Pei Liu,% }0 N0 p u7 y7 X2 l3 r
and Sheng-He Huang .................................................................... 301: O9 G8 L9 a, d! j; Q
32 Gene Cloning and Expression Profiling by Rapid Amplification; I% a2 ?9 g0 v1 Y. V( n
of Gene Inserts with Universal Vector Primers. p2 S6 \2 X. R* s" p6 @ V/ d
Sheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong .............. 309
+ T/ N; B* D) R1 @9 }5 I- |& }33 The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions
/ `6 l( |6 n5 W! O( fby Inverse PCR
1 `/ v! l {- T( QVincent J. J. Martin and William W. Mohn ...................................... 315) E( g. z' i% b5 f9 |& ?3 j
34 Rapid Amplification of Genomic DNA Sequences Tagged# w* V; ^# h) d" |3 W
by Insertional Mutagenesis; k) J Z; L# m3 M) c% K1 W' G
Martina Celerin and Kristin T. Chun ................................................ 325& K- s4 A4 U h# C
35 Isolation of Large Terminal Sequences of BAC Inserts Based
" P6 _" O' g4 A% ~on Double-Restriction-Enzyme Digestion Followed
# \( l5 t% D# h8 U# e; Wby Anchored PCR
+ I. N: U! }4 u" S/ cZhong-Nan Yang and T. Erik Mirkov ............................................... 337; f8 R3 \( k" @* l- R/ W
`; O$ b* a# _) @1 c$ Y& A8 ~36 A “Step Down” PCR-Based Technique for Walking
& y% M% j! u+ H3 }Into and the Subsequent Direct Sequence Analysis) c( N9 j( ^: y$ N% Y2 n+ }0 F
of Flanking Genomic DNA. l' N. y9 J: W( [' t8 Z" v$ K6 P
Ziguo Zhang and Sarah Jane Gurr .................................................. 343& n- m0 A6 C. z3 t+ f1 y7 F
PART V. LIBRARY CONSTRUCTION AND SCREENING$ E/ W1 c5 x5 R" t- n$ O2 j
37 Use of PCR in Library Screening: An Overview
- T" h1 D n9 n4 v- f& xJinbao Zhu .......................................................................................... 3532 Q( K1 K8 d, D, m
38 Cloning of Homologous Genes by Gene-Capture PCR% ~ G' q( i, v+ r) T( i. d( A
Renato Mastrangeli and Silvia Donini ............................................. 359# R( Q, B; k M4 Q2 C2 k
39 Rapid and Nonradioactive Screening of Recombinant Libraries by PCR
' b- Y. Q! T( t G( a% Z" lMichael W. King ................................................................................. 377
9 L6 ]7 Z% Q* l+ |& t40 Rapid cDNA Cloning by PCR Screening (RC-PCR)
- }5 T* \9 [" k1 Z4 o# N+ IToru Takumi ....................................................................................... 385; @) |, Y( T- S
41 Generation and PCR Screening of Bacteriophage λ Sublibraries
. c7 o! l7 V3 ^2 |4 U# L0 O2 dEnriched for Rare Clones (the “Sublibrary Method”)
/ ?1 G0 q4 v+ e. m1 N: }+ f* TMichael Lardelli .................................................................................. 391
6 a# t7 X# N' a2 |, T- O42 PCR-Based Screening for Bacterial Artificial Chromosome Libraries/ V% y# s( ^& m8 q2 r
Yuji Yasukochi ................................................................................... 4015 B2 v- e5 M/ I: e6 ~
43 A 384-Well Microtiter-Plate-Based Template Preparation) o* |9 @) z' M, G4 q8 X0 i
and Sequencing Method
4 s5 R. ]; B3 \, {+ |6 x3 e9 S% CLei He and Kai Wang ......................................................................... 411
6 f4 l. |, U, [0 ~$ E8 D% t44 A Microtiter-Plate-Based High Throughput PCR Product
* U# b; Z: l! P* `- o' e* h$ rPurification Method" R% `# [- C6 N( _! A o
Ryan Smith and Kai Wang ................................................................ 417
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发表于 2011-6-11 11:43
发表于 2011-6-11 14:46
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