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本帖最后由 细胞海洋 于 2014-10-8 14:01 编辑 . I( l6 q! ^8 Y- I; n/ R
! A o, u8 ?7 Q# u肿瘤是由形态和分子迥然不同的细胞构成的嵌合物。在这样的细胞异质性中，癌症干细胞只占据了肿瘤块的1-2% ，在过去的数年里，科学家们提出癌症干细胞是癌症的起源，并导致对了传统化学疗法耐药。由于在实体瘤中癌症干细胞只占很小一部分，使得分离出它们并对其进行分析，研究耐药的根源变得特别的困难。5 S5 e8 ~2 n+ ^
4 Y7 c, A4 N. m. {% G; A, G4 O9 p来自西班牙国立癌症研究中心(CNIO)的研究人员，发现癌症干细胞一种新的特异标记物：核黄素（维生素B2）,并确定了其特征。核黄素是一种色素，随着它在细胞内囊泡中累积会发出绿色的荧光。无需抗体或其他更加昂贵和复杂的技术，可利用这一发光特性来追踪、分离以及随后纯化出癌症干细胞。" Y: B1 k2 [9 i' F) R- h7 r
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由科学家Irene Miranda、Bruno Sainz和Christopher Heeschen领导的研究小组，将他们的研究结果发布在9月28日的《自然方法》（Nature Methods）杂志上
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' L- x* y% u `Intracellular autofluorescence: a biomarker for epithelial cancer stem cells
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Cancer stem cells (CSCs) are thought to drive tumor growth, metastasis and chemoresistance. Although surface markers such as CD133 and CD44 have been successfully used to isolate CSCs, their expression is not exclusively linked to the CSC phenotype and is prone to environmental alteration. We identified cells with an autofluorescent subcellular compartment that exclusively showed CSC features across different human tumor types. Primary tumor–derived autofluorescent cells did not overlap with side-population (SP) cells, were enriched in sphere culture and during chemotherapy, strongly expressed pluripotency-associated genes, were highly metastatic and showed long-term in vivo tumorigenicity, even at the single-cell level. Autofluorescence was due to riboflavin accumulation in membrane-bounded cytoplasmic structures bearing ATP-dependent ABCG2 transporters. In summary, we identified and characterized an intrinsic autofluorescent phenotype in CSCs of diverse epithelial cancers and used this marker to isolate and characterize these cells.$ }* Q5 y/ A, `, m5 Y7 M g: r4 D
% ^4 z, q' {3 D f# [6 G$ Y6楼原文 感谢zcysgdtc 提供 |
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发表于 2014-10-1 12:39
发表于 2014-10-2 00:26
