PDF电子书：Protein expression in mamallion cells （Humana Press 2012）

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1 E2 P% `$ w3 x" v9 Y( S  M9 q METHODS   IN  MOLECULAR  BIOLOGY
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5 `  d" O  p; j3 FProtein Expression in Mammalian Cells
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0 B1 p6 s7 u; n5 XMethods and Protocols  {: {* z  L: P3 _/ \# W
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Edited by
8 g: z% O& R" v, Y% sJames L. Hartley
5 ?" H$ s+ y9 H( ~- Y" JProtein Expression Laboratory, SAIC-Frederick, Inc., National Cancer Institute, : T$ U+ l- W" F. @0 d8 ?
Frederick, MD, USA
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Springer Science+Business Media, LLC 2012. `# \) [, U( M% C- _* k; D
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Contents/ g: X( k; K9 ]. s1 J$ B
Contents
& w/ c6 y( U$ y3 BPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  v
9 ?; \" g. D7 ~& }4 ~: PContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  ix: z: l* `& g, b& i' r
  1  Why Proteins in Mammalian Cells? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1- t& f! F! r/ q: _! E' W, {
James L. Hartley
5 `# A' k$ D, b  2  Large-Scale Transfection of Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  13
5 H. u. ]9 l5 q  t  r" ELucia Baldi, David L. Hacker, Carine Meerschman,
  j1 s+ I8 d. xand Florian M. Wurm
! `: u+ |  ]4 m) C  3  Selection of High Expressing Mammalian Cells by Surface Display 0 l  t8 s$ D9 V: w
of Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  27' s  i3 K' i0 G: T' h
Christine T. DeMaria1 v' \# M* c" M5 G# y- \
  4  Expression of a Secreted Protein in Mammalian Cells Using
! z; Y  z+ ~! C1 T$ _0 y2 OBaculovirus Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  41
/ `( v* u7 \) P  OBarbara Ann Jardin, Cynthia B. Elias, and Satya Prakash
% q  d9 C% A0 A. V) L5 Y9 A8 Z  5  Transfection of Difficult-to-Transfect Primary Mammalian Cells . . . . . . . . . . . . . . .  65
9 }# {4 L2 c& t/ IOliver Gresch and Ludger Altrogge
. n/ }2 q. \* t0 O+ i  Q  6  Stable Protein Expression in Mammalian Cells Using Baculoviruses  . . . . . . . . . . . .  757 P% t6 n9 m) J+ @( q: r
Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty,
/ C: ~0 t# Z8 h5 S5 _, g; o2 g9 USabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch) |6 s% `6 y9 R' z' l
  7  Using Matrix Attachment Regions to Improve Recombinant & O% C5 B/ j6 M( R$ I+ b3 n, z
Protein Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  93
5 p$ Z0 \2 Y3 F3 J' \3 w8 rNiamh Harraghy, Montserrat Buceta, Alexandre Regamey, 4 s" A! {+ B, K5 k9 ]! H+ k
Pierre-Alain Girod, and Nicolas Mermod
" D4 V$ _) z  ^* X4 F  8  Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells  . . . . .  111: G" p% ?6 [+ G* W, S
Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, + F# Y: G# b7 _" m8 ?7 d7 F# ]) l3 z
and Tina M. Sauerwald
( e8 m. v- x/ Y5 g3 n: M( |  9  Post-transcriptional Regulatory Elements for Enhancing Transient Gene
7 H% J/ s; `2 rExpression Levels in Mammalian Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  125
% b  `8 `+ r: [% v9 v0 n! _$ P! cMariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang
. [9 M9 ?, L6 v+ ]0 v0 Y10  Converting Monoclonal Antibodies into Fab Fragments for Transient
# H# Y2 E% x- z9 i* Y% DExpression in Mammalian Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
* N0 f) @( q/ LJoanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq,
2 k+ P( [1 H- R2 l# ~( f" L0 {, IRebecca Hamer, and Raymond J. Owens
' X7 _  w$ \, `, e- C, d11  Generation of High-Expressing Cells by Methotrexate Amplification ) p3 R+ q% E7 \# T8 T  i. t# T' M- m
of Destabilized Dihydrofolate Reductase Selection Marker  . . . . . . . . . . . . . . . . . . .  161
9 M: s+ w; s* K! I0 ]Say Kong Ng
" \4 k" J/ I# a: i& e12  Tools for Coproducing Multiple Proteins in Mammalian Cells  . . . . . . . . . . . . . . . .  173' W# {2 @" H# z- t8 P7 o  P, ?
Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia% }  f! z9 w8 G
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13  Identification and Characterization of Protein Glycosylation Using Specific
* D( n/ O1 _  w& }7 f) u+ B* q9 ?Endo- and Exoglycosidases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  189; M; _* y0 d8 }  e: z
Paula Magnelli, Alicia Bielik, and Ellen Guthrie/ O# O1 T' [1 |/ _% v% t8 x
14  Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA  . . . . . . . . . .  213% a7 Y% }. I, V5 U9 {* Y
Renate Kunert and Karola Vorauer-Uhl/ K3 I5 p) G" X% q  \
15  Methods for Constructing Clones for Protein Expression in Mammalian Cells  . . . .  227
" m& s8 M$ @0 p! T4 S# STakefumi Sone and Fumio Imamoto& e) l  l/ [1 M' h  H- e
16  Optimizing Transient Recombinant Protein Expression in Mammalian Cells . . . . . .  2519 P: o- B+ X; C/ B* [  m
Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito7 s+ K4 u$ H7 Y
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  269* K" ?6 [9 f  u1 Z8 ^/ a$ v
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mimimi2256

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Why Proteins in Mammalian Cells

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cc1986

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