构建造血干细胞源性诱导多能干细胞的抗HIV-1功能巨噬细胞

botizhou

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sunny2829

是不是这篇啊？？有人发过了~~~+ l& ?$ \$ q" q+ g9 z+ U! {
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诱导的多能干细胞（iPSCs）为艾滋病的治疗提供了希望/ K7 w, |! h  ]" E8 d5 L
http://www.stemcell8.cn/thread-36638-1-1.html
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( F8 T- y: H3 x7 w; oMol Ther. 2011 Mar;19(3):584-93. Epub 2010 Nov 30.
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9 H) H4 T4 S9 w& UGeneration of HIV-1 Resistant and Functional Macrophages From Hematopoietic Stem Cell-derived Induced Pluripotent Stem Cells.
7 Y  i' }9 c  i6 N7 \. a$ bKambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS.
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Stem Cell Program, Department of Internal Medicine, University of California, Davis, Sacramento, California, USA.: C/ i- ~2 L$ ]
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Abstract
1 \8 o" t2 b4 w+ v: }! KInduced pluripotent stem cells (iPSCs) have radically advanced the field of regenerative medicine by making possible the production of patient-specific pluripotent stem cells from adult individuals. By developing iPSCs to treat HIV, there is the potential for generating a continuous supply of therapeutic cells for transplantation into HIV-infected patients. In this study, we have used human hematopoietic stem cells (HSCs) to generate anti-HIV gene expressing iPSCs for HIV gene therapy. HSCs were dedifferentiated into continuously growing iPSC lines with four reprogramming factors and a combination anti-HIV lentiviral vector containing a CCR5 short hairpin RNA (shRNA) and a human/rhesus chimeric TRIM5α gene. Upon directed differentiation of the anti-HIV iPSCs toward the hematopoietic lineage, a robust quantity of colony-forming CD133(+) HSCs were obtained. These cells were further differentiated into functional end-stage macrophages which displayed a normal phenotypic profile. Upon viral challenge, the anti-HIV iPSC-derived macrophages exhibited strong protection from HIV-1 infection. Here, we demonstrate the ability of iPSCs to develop into HIV-1 resistant immune cells and highlight the potential use of iPSCs for HIV gene and cellular therapies.
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dypnr

干细胞研究还要面向临床

榴榴莲

干细胞研究非常有前途

我心飞翔

免疫细胞疗法治疗肿瘤有效  

sky蓝

嘿嘿  

nauticus

干细胞存储  

tuanzi

我也来顶一下..  

123456zsz

彪悍的人生不需要解释。  

剑啸寒

加油啊！！！！顶哦!!!!!