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本帖最后由 细胞海洋 于 2013-1-12 22:42 编辑
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+ ~1 K/ ~- g& R4 k" f- D) y$ FRibosome display and related technologies : methods and protocols 7 m$ |+ E5 T0 a4 D2 A8 T! {8 W
edited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805) ( L9 T6 \0 i: l0 W
5 m- ~! G/ }5 w4 H Contents
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Preface
$ [6 m9 l' g$ K* O2 [ Contributors
4 M- h8 P3 Y* R+ Z# _ PART I REVIEW3 v/ m1 M3 x# F/ y" v% R
1 Ribosome Display: A Perspective' O; W6 E: ~* V5 ?& t' x# n
PART II TRANSLATION EXTRACT PREPARATION
z) [& x5 E- _! c; N 2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts+ G' y3 ~ x( Y" G. ^8 C, s
PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS8 K) E- f2 U J
3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 45) U' h- ^4 [; s
4 Stabilized Ribosome Display for In Vitro Selection; R- p9 {' C- H
5 Eukaryotic Ribosome Display with In Situ DNA Recovery 75
# V$ ?* [5 G; n, R 6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 878 o" z4 ?3 H Y( ?* u0 K
7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101 f5 @; A/ Y0 c! ^+ q5 n$ Q1 w9 {( ^
8 cDNA Display: Rapid Stabilization of mRNA Display 1131 I. z! x# b+ k, I0 E% K$ W
PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS. u" q, b! \8 n6 D; g
9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
& f& T7 @2 F# f* @3 }1 h6 E y7 L 10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display8 z& s/ P# ] ]
11 Evolution of Protein Stability Using Ribosome Display
* `" H$ q7 @0 @: V5 l 12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries1 h. k; v k/ u: T) |
13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
! |' O2 P; G. N 14 Peptide Screening Using PURE Ribosome Display
9 F2 I& @6 ?: O 15 Rapid Selection of High-Affinity Binders Using Ribosome Display 261
. @4 c3 ?! z/ }( _ 16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287. e/ c- k8 ^3 T1 w: _0 w- s
17 Identification of Candidate Vaccine Genes Using Ribosome Display 299
# n" _. S# o$ P8 P7 g( n 18 Ribosome Display for the Selection of Sac7d Scaffolds 3155 B( a0 m+ U& V% S
PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS
7 q- P- r1 L' z* I 19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335
% `8 R- Z9 `: Y% h 20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349* _$ u ]$ s/ R' B, F
21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 367) E8 q% [8 P O/ F' w+ Z C
PART VI CASE STUDIES7 R# {$ @" j% ~* \
22 Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display" C+ P7 j; l) j0 e9 o; T0 T
23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 403; m7 f9 Z. e" J' s. A# Y
Index 423
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