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《Bovine CD49 positive-cell subpopulation remarkably increases in# Q0 M4 T+ A9 W; f( _" N& Y
mammary epithelial cells that retain a stem-like phenotype》---Research in Veterinary Science
/ a& \# m3 R- [6 \4 H- R' rWe previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential, W! U- e- _+ _2 b! W
to generate a functional mammary outgrowth. The aim of this study was to investigate on the0 x7 q9 |9 z; w$ d! ]2 Z
immunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry1 k; W. a. |6 G# n a1 K
analysis showed different subpopulations of mammary epithelial cells emerging according to the timing of$ b. v( H) C: M3 h3 j1 u
cell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed,
% ]1 B) b# M' z( G6 |5 B* Eeven if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed- G# ~3 `& U3 y( m. a, u1 H, g
for CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative3 r% i( ~+ I- q9 Q5 B# n5 [+ r
capabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when
. U8 {. D, a* A5 ^( N3 qtransplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation
0 F% p' R' ?# k9 d$ c% \3 \that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion% b5 C* z: C W* y }
of the primitive population of adult mammary stem cells. |
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