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- 积分
- 2199
- 威望
- 2199
- 包包
- 5384
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1000X FIAU [1-(2'-deoxy-2'-fluoro-b-d-arabinofuranosyl)-5-iodouracil]: 3 L; U1 b$ K) G3 X1 `( [
Obtained as crystalline powder from Bristol Myers (patent now owned * \5 H: v3 e, j* e& b
by Oclassen Pharmaceuticals, Inc.) and stored in a light-proof,
+ T l3 m# D) |# j0 p5 D$ e, pbrown bottle @ 4o C in T932. C9H10N2O5FI FW=372.08 (molarity
/ v e' w' ^% s( ?4 _) s; ccalculations based on an estimated FW of 388).
, K& M( Z; |4 ?2 ]' V( a. ^
2 `$ t' ~+ y2 C. x" t3 V( ` The 1000X stock solution is prepared in PBS (alkalinated % s( J% G( [6 X/ H U: g
with NaOH) to give a final stock concentration of 200 mM.) c7 {8 l8 e, O& {! t9 n ~
7 F4 f" l( H( o# ^% q5 ?$ L
Procedure:: g# i1 m) n/ w; i- j, j4 n7 |
& Z4 T% C! s% Y7 ?8 o-- Prepare an intermediary 100 mM stock solution by adding 388 mg $ G U% X& V: d: ?/ j# x
of FIAU to 8 ml of PBS. To get the FIAU completely in solution, add
1 d5 m" }7 I4 Q- e5 N NaOH dropwise until the powder is fully dissolved. Once dissolved, ( Y `$ P* H3 }6 k4 C
bring the volume to 10 ml with PBS. Divide into 1 ml aliquots and store
& G: J$ L, o7 `1 y* ^- x$ J@ -20o C. o3 R' E# ~9 q/ b# G, t; K' W2 v
: `2 c9 ^# |5 `# }/ d% v8 L-- When needed, prepare stocks of 1000X FIAU by adding 100 ml of
, W2 g3 ]' r/ |" I0 z! \) O! vthe thawed 100 mM intermediary stock FIAU to 50 ml of PBS. If the
" j7 s8 s9 n# P/ q* D. o% oFIAU precipitates, add 5 N NaOH dropwise until the FIAU goes back ( W6 h% m# e; t. m1 \
into solution.
9 s4 U* [* \) y4 {0 d: D
8 X# K6 X$ f0 I" Y- G9 c( Y-- After mixing, filter sterilize the 1000X FIAU through a 0.2 mm ) f! i7 a, n; r! Q9 W" t; g- w
syringe filter, aliquot into sterile freezing vials, label with
g; w, r, Z- W"1000X FIAU", and store @ -20o C.4 y5 J ^5 O8 N4 r1 V+ P" \% l
2 Y) ~9 b, @1 {' f
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总评分: 威望 + 10
包包 + 20
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发表于 2012-3-26 11:39
