慢病毒转染的原理及过程（个人理解，求解惑）

第一心音

我们也用的293T，其他种类的细胞我也用过，不好，建议不要用，还有，你的思路很清晰，过程正确

芦苇浅

回复 shihuijunm 的帖子, l) `/ {  D9 J6 [; ]: T& K/ Z1 v

0 }# _3 \, Z. j( C# |( p- {The 293FT Cell Line, a derivative of the 293F Cell Line, stably and constitutively expresses the SV40 large T antigen from pCMVSPORT6TAg.neo and must be maintained in medium containing ; c2 E: Y- ?  m# R0 I7 d3 j1 X! h
Geneticin. So it's different from the 293 T cell line.
2 Y/ p0 l! X7 l, i' r$ p$ IBest regards!

芦苇浅

回复 greenjing 的帖子
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There are so many factors  influncing your transduction efficiency:1. MOI too low; 2. Too much antibiotic used for  selection; 3. Cells harvested too soon after  transduction; 4. Gene of interest is toxic to cells; 5. Non-dividing cell type used etc.

yiyi8909

回复 zerollx 的帖子
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好像看有的说F的意思就是fast，生长速度比293T快，它们两个都是整合了SV40大T抗原。具体的也不是很清楚。( S2 s7 |( w' V9 a
The 293FT Cell Line is derived from the 293F Cell Line and stably
7 z! O3 W! {( k/ ?! s1 l9 R( P) [/ ~expresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid.+ v5 f' d& V0 r& i3 [
Expression of the SV40 large T antigen is controlled by the human cytomegalovirus
' c& O- ]  ]6 h(CMV) promoter and is high-level and constitutive. Studies have demonstrated maximal virus production in human 293 cells expressing SV40 large T antigen (Naldini et al., 1996), making the 293FT Cell Line a particularly suitable host for generating lentiviral constructs using the ViraPower Lentiviral Expression System available from Invitrogen.- P7 z+ o+ W4 [6 U
The 293 Cell Line is a permanent line established from primary embryonal human1 X3 A5 d* p4 w7 V" r5 ?& A
kidney transformed with sheared human adenovirus type 5 DNA (Graham et al.,
9 H$ W* L0 y& L# k0 K1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells6 L6 B) G5 L5 W6 }% X
and participates in transactivation of some viral promoters, allowing these cells to
; M7 T* u: p9 M" d7 J( o7 lproduce very high levels of protein.+ C' v! \7 b. ^0 q& b+ f
The 293-F Cell Line available from Invitrogen (Catalog no. 11625) is a fastgrowing
$ P' J! I* ?) \  q! o1 f0 Y# V$ a  Hvariant of the 293 cell line, and was originally obtained from Robert Horlick at Pharmacopeia.
1 E' L) h& [7 P3 p" L  q' h这个以供参考！

thinktank

不错。' z* [1 V4 M+ G! a

小兔子lp

如果不包装病毒，直接用脂质体2000转染目的细胞，比如MEF，结果会怎样呢？目的基本是不是也能转染到MEF中去？

细胞海洋

回复 小兔子lp 的帖子$ |. U$ a  v. ^1 |

  {1 Z& n# U+ t建议你发新帖提问

qinrui

你好

qinrui

你好

温宗壮

实用，多谢