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最近看文献,文献中这段话我看了几遍不知道什么意思,虽然自己之前是学生物的,对这方面接触的还比较少,想请高手帮我看下下面这段是讲什么的?什么意思,万分感谢!
) \6 u3 s6 l1 Z7 N! wThe CMV promoter element was removed from the FLAG tagged tetrameric wild-type EosFP by restriction digest with BglII/KpnI and the annealed and phosphorylated oligos of the miniCMV promoter sequence (bp 51 to +77 of the CMV promoter) were inserted. This construct was then digested with ZraI to allow insertion of the annealed, phosphorylated and ligated HRE element oligonucleotides. Confirmation of HRE copy number was confirmed by digestion with KpnI/SspI/XbaI andthrough DNA sequencing by the Australian Genome Research Facility . |
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