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- 积分
- 266
- 威望
- 266
- 包包
- 863
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O
, K* h2 }- L4 [& y(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g( G& B5 \, d T m2 J) z: m( ~
MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),
x" O4 `$ ]6 f0 q4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;3 M- Q9 G/ j Q
Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.9 `5 [% P# U9 o' P: o
G6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g
, s0 u- o- S0 O" @penicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate6 \' T- e8 I9 N* t+ X
(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of
+ S1 z/ P* s0 k9 ]: R1 q, jMilli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L2 B0 _9 ^, ^0 i4 ^8 d- q& }5 A8 {% x
with Milli-Q water. |
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发表于 2009-9-21 09:25
发表于 2009-9-26 07:48
