M2培养液

POPOLD

M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O
/ ^! g8 W* E9 L$ h* ~, w3 W(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g) _$ ]. Z5 [0 n1 O* M" L- u$ y
MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),, t2 g/ U+ ]. M' s
4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;
9 t7 Y, q4 R& K  {" N& N9 H9 qSigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.8 @2 m  C2 j' q6 V: s" H. H+ J0 X
G6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g
6 O  J$ i; |) C5 I0 q( V) }' R5 ypenicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate
7 ?3 g, ]8 Z, U& y& v(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of& G  C+ S7 ^0 j- S) q
Milli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L+ F0 c* X+ i- `/ G
with Milli-Q water.

zpzp0312

谢谢！~~~

quixote1983

还有编号，太职业了

POPOLD

您的 满意 ，是版主最大的希望，还可亲临指导

jayhaoli01

谢谢！

hualin840518

回复 1# POPOLD
# Y, e/ J. K* }- H3 v2 m& \/ u
$ L8 P' q# x0 Z" v# D& L: @/ G4 R6 V, L) e% F7 T8 u1 e* F: f: V- |- \
    来源是那篇文章
# R9 a  |% C) @. f谢谢！

edisonlou

谢谢

llingzu

谢谢

基拉

谢谢

POPOLD

回复 12# hualin840518 0 g4 }/ E1 N7 Z3 V
0 G; O9 `% h- v1 |0 d

# u# u8 V! J1 T, o5 e5 j    yige 一个牛核移植的protocol