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回复 zerollx 的帖子2 ~4 Y7 t* T5 }3 S* n9 N+ D
) i- N3 @' N( |; c1 n好像看有的说F的意思就是fast，生长速度比293T快，它们两个都是整合了SV40大T抗原。具体的也不是很清楚。
. k3 D: s6 }# d; ~; o7 gThe 293FT Cell Line is derived from the 293F Cell Line and stably: z; V7 g, n5 X# f Q
expresses the SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid.
3 Z; H5 I$ k# `. _Expression of the SV40 large T antigen is controlled by the human cytomegalovirus
8 V. c0 P6 W0 V0 t) I$ U9 z; U(CMV) promoter and is high-level and constitutive. Studies have demonstrated maximal virus production in human 293 cells expressing SV40 large T antigen (Naldini et al., 1996), making the 293FT Cell Line a particularly suitable host for generating lentiviral constructs using the ViraPower Lentiviral Expression System available from Invitrogen.
, e. _% a1 D( s# MThe 293 Cell Line is a permanent line established from primary embryonal human' ] H* f1 z0 h1 ^
kidney transformed with sheared human adenovirus type 5 DNA (Graham et al.,
1 I; E: J8 |9 x ]# J( q1 _5 d& T1977; Harrison et al., 1977). The E1A adenovirus gene is expressed in these cells! _6 }- ] e) G. u$ }' c$ o
and participates in transactivation of some viral promoters, allowing these cells to+ Y% F; f% j2 \7 h9 H
produce very high levels of protein.
M; L1 }4 t& X! TThe 293-F Cell Line available from Invitrogen (Catalog no. 11625) is a fastgrowing6 E u7 z/ I. Y' d, j
variant of the 293 cell line, and was originally obtained from Robert Horlick at Pharmacopeia.- O: w3 i' D% W) S2 C/ d- D1 L
这个以供参考！ |
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发表于 2012-10-12 20:44
发表于 2014-8-15 08:29
