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本帖最后由 flowguo 于 2012-10-11 10:53 编辑 0 Y8 Q! P7 {) o# X- [( L j5 Z
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Dendritic cell protocols
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: e* B% f3 }) `$ ~3 Z. Y# Xedited by Shalin H. Naik. — 2nd ed.
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Foreword
6 S4 p& ]% J: P Contributors i. `8 u" K5 U9 g
SECTION I REVIEWS
4 R' a: z2 M8 O" k 1. Review of Human DC Subtypes
6 b, ^" i0 F8 N) W `% }4 H 2. Review of Murine Dendritic Cells: Types， Location， and Development 219 Z" K, A& L# L
SECTION II HUMAN DC PROTOCOLS 43
5 I) x; A6 G. E, ?/ t 3. Isolation of Human Blood DC Subtypes* F$ B) \7 j/ B1 _3 P7 F: B3 P
4. Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin 55" S# n0 Y* ^* o6 p. a6 a
5. Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells 67
* }! Y! c: R* P% I7 V/ z, y 6. In Vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2-/-%-/- Mice8 N" s" F& W5 Q1 Z% G
7. Generation of Stable Th1/CTL-， Th2-， and Thl7-Inducing Human Dendritic Cells
1 k% S# `" {5 g: z, X+ ^+ |: O 8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation
, Q5 n, I5 M' @, I 9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines 149" \% j5 N% b' \+ o o: f2 W
SECTION III MURINE DENDRITIC CELL METHODS 165
5 W+ I, f. h, q( X1 V( S9 U 10. CD8+， CD8-， and Plasmacytoid Dendritic Cell Generation In Vitro Using fit3 Ligand 167
3 ?! f: o* a# j( M5 O- ~$ b( | 11. Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro 177
( T8 X G6 B* x& B2 F" O9 B 12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF 187) f+ y w( K: A7 H% a+ t/ z
13. Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow 195
. O0 ?; M x+ J7 I 14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry 205
+ M: W+ {- g l2 ~ 15. Isolation of Cutaneous Dendritic Cells 231
9 b( l4 U, Y7 j& n- O7 A8 t 16. Isolation of Skin Dendritic Cells from Mouse and Man 235( B7 a2 d {8 m) H
17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells 249* B$ F# X+ O9 E. l M! K& e- [5 l
18. Isolation of Mouse Thymic Dendritic Cell Precursors 2659 @8 w6 F- y/ u# l
19. Isolation of Rat Intestinal Lymph DC 281' F$ |+ {" \4 ~* `
20. Mouse Models of Viral Infection: Influenza Infection in the Lung 299
7 f: o- \3 _0 h: R 21. DCs in Mouse Models of Intracellular Bacterial Infection 319
, l. D4 t/ H3 i& i! w4 E' c( b 22. Studying the Function of Dendritic Cells in Mouse Models of Asthma 331 S0 p! ]7 R$ v7 B
23. Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation 351
7 C! J- r) s! o0 @+ c% @ 24. Methods to Study Pulmonary Dendritic Cell Migration
' T4 J) N: A" L8 N; ^3 N z# k 25. Measuring pH， ROS Production， Maturation， and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays 383
$ K3 L1 N) f, L6 o% ~ 26. Adaptive Treg Generation by DCs and Their Functional Analysis 403
- |! ]3 P4 Z2 U! Y 27. Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation 413) H+ ^) Q [( a6 m
28. Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice+ y4 J5 `0 Y9 q9 z6 s0 t# F% Q( K2 v" D
Subject Index 443
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发表于 2012-10-11 10:49
发表于 2012-10-11 13:03
