Nat.Methods：科学家研发出培养多能干细胞新系统

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研究人员研制出了一种简化的、化学成分明确的培养系统，可用于人类多能干细胞的生长，新成果发表在日前在线出版的《自然—方法学》期刊上。新方法很重要， 既可用于标准化来自不同实验室的报告结果，也可最终用于这些细胞的临床应用。
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8 I  m! b0 H7 s3 {8 O多能干细胞的研究部分属于科学，更多属于艺术，因为它们对培养环境有高度的敏感性：如果没有被恰当处置，它们或者会死亡或者失去其“干细胞性”。过去，科学家们的许多工作是努力定义出让干细胞生长的混合培养液的相关成分。! }4 K" H( r6 h1 A

4 k( z" t4 V" {% B, \7 ?James Thomson和同事报告了对一种干细胞培养液的系统剖析，定义出它的基本成分。在一项辛苦的工作中，他们挑选出液体培养液中的8种关键成分，并在化学上定义出这些成分。他们还定义出表面涂层的成分，指出这些成分既能让培养液长时间高效地培养人类胚胎干细胞，也能长时间高效地培养来自新鲜活组织切片的人类诱导多能干细胞。（生物谷Bioon.com）
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2 v  R0 C; m9 H; f, d% p生物谷推荐原文 出处：. A8 ?4 I' n0 U+ K& u0 l7 l

2 W# H0 }/ D7 @1 ^Nature Methods    DOI:10.1038/nmeth.1593. d$ u: @% |8 @1 X9 L% Q
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Chemically defined conditions for human iPSC derivation and culture
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6 h4 q3 d+ I+ T" R0 hGuokai Chen; Daniel R Gulbranson; Zhonggang Hou; Jennifer M Bolin; Victor Ruotti; Mitchell D Probasco; Kimberly Smuga-Otto; Sara E Howden; Nicole R Diol; Nicholas E Propson; Ryan Wagner; Garrett O Lee; Jessica Antosiewicz-Bourget; Joyce M C Teng; James A Thomson
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We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods.( T, U: [/ X( a

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