小鼠胚胎成纤维细胞能够传多少代？

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Root apical papilla was gently separated from the surface of the root, minced and digested in: b! ^  [. E) \' j; B  r& M& V
a solution of 3 mg/ml collagenase type I (Worthington Biochemicals Corp., Freehold, NJ) and
; k8 j* W$ F  h; r  V9 G4 mg/ml dispase (Roche Diagnostic/Boehringer Mannheim Corp., Indianapolis, IN) for 308 C% y0 `5 S! t2 n: f
minutes at 37°C. Single cell suspensions of SCAP were obtained by passing through a 70 μm) k% k) c# K. S! w; i
strainer (Falcon, BD Labware, Franklin Lakes, NJ), seeded at 1×104 into 10-cm culture dishes
8 q" D: Y" \) ?* b8 ?8 T4 p(Costar, Cambridge, MA), and cultured with α-Modification of Eagle’s Medium (GIBCO/
( D2 k  \, ~0 l9 OInvitrogen, Carlsbad, CA) supplemented with 15–20% fetal bovine serum (FBS) (Equitech-: B; ?/ U7 `) G( H9 G1 w
Bio Inc., Kerrville, TX), 100 mM L-ascorbic acid 2-phosphate (WAKO, Tokyo, Japan), 2 mM7 u( d' J2 A3 M" W1 i4 l& b
L-glutamine (Biosource/Invitrogen), 100 U/ml penicillin and 100 mg/ml streptomycin at 37°
' \0 |5 `" k/ i8 \/ nC in 5% CO2. 这是我要养的这个间充质干细胞的培养方法，我想问的是：1：a-MEM和DMEM的区别，当然除了成分上的区别，是不是一个促进细胞的分化？有的人养牙髓干细胞会用GIBCO DMEM/F12(1:1) 11330，有的用hyclone的SH30265.01B。2:100 mM L-ascorbic acid 2-phosphate 这个L-抗坏血酸2-磷酸酯的具体的作用是什么呢？提供vc的营养，只是比vc稳定？可是矿化诱导液里面是加了抗坏血酸的，这不是促进干细胞的分化嘛？3：我按照文献里，查到http://www.wako-chem.co.jp/english/labchem/productinf.htm 这个公司的产品只能查到L-Ascorbic Acid Phosphate Mg Salt.nH2O。sigam公司也只能查到A8960 SIGMA  L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate 。我到底该怎么办呢？这个细胞的培养 我到底该选择什么样的培养基，这个L-抗坏血酸2-磷酸酯 我该怎么买呢？