2011年6月的国际学术期刊《干细胞》(Stem Cells)发表了中国科学院深圳先进技术研究院医工所王立平和蔡林涛研究组的研究论文,他们在锶促进间充质干细胞成骨分化及掺锶材料修复骨缺损的效果和机制研究中取得新成果。本项研究的部分成果已同时被国外Mason RS研究组证实(Journal of Biological Chemistry, 2011年5月12日)。9 E* J' H/ W. B
! ~5 w& z8 w+ v O+ L, B该工作由杨帆、杨大志、屠洁等共同完成。此研究工作的掺锶原材料由香港大学医学院骨科系吕维加教授(W. William .Lu)提供。8 c; Q* ]0 ^& y* s4 Q! R
8 t, a, B$ I; iStrontium Enhances Osteogenic Differentiation of Mesenchymal Stem Cells and In Vivo Bone Formation by Activating Wnt/Catenin Signaling 4 H: D1 F5 N3 u4 x9 |; K/ ~& [3 u / {- S5 H: F6 Z/ a5 {% SYang, Fan; Yang, Dazhi; Tu, Jie; Zheng, Qixin; Cai, Lintao; Wang, Liping 9 a& G8 ?2 T( c/ e1 s " x% n0 O5 ~/ R+ L- zKeywords:Strontium;Mesenchymal stem cells;Wnt/β‐CateninAbstractStrontium ranelate is a newly approved drug that can reduce the risk of vertebral fracture, which is attributed to its dual function in increasing the bone formation and decreasing the bone resorption. Strontium‐containing hydroxyapatite was also demonstrated to stimulate the osteoblast activity and inhibit the osteoclast activity. However, the molecular mechanisms of strontium underlying such beneficial effects were still not fully understood. In this study, we investigated the effects of strontium on the osteogenic differentiation of human mesenchymal stem cells (MSCs) and its related mechanism; its osteogenic potential was also evaluated using a calvarial defect model in rats. We found that strontium could enhance the osteogenic differentiation of the MSCs, with upregulated extracellular matrix (ECM) gene expression and activated Wnt/β‐catenin pathway. After transplanting the collagen‐strontium‐substituted hydroxyapatite scaffold into the bone defect region, histology and computed tomography scanning revealed that in vivo bone formation was significantly enhanced; the quantity of mature and remodeled bone substantially increased and ECM accumulated. Interestingly, strontium induced an increase of β‐catenin expression in newly formed bone area. In this study, we showed for the first time that strontium could stimulate the β‐catenin expression in vitro and in vivo, which might contribute to the enhanced osteogenic differentiation of MSCs and in vivo bone formation. STEM CELLS 2011;29:981–991 ' I- `6 M, r" y; d& ]# D7 V % `- ^8 Q0 u- c6 D